Application description. BRAND NAME. Low-pressure chromatography columns, in sizes 50 to 200 mm inner diameter (i.d. T: +44(0)1357 522961 F: +44(0)1357 522168. Hyperclone ODS (C18) Hyperprep HS C18. The current United States Pharmacopoeial Convention (USP) method for Atropa belladonna extract specifies a gas chromatography (GC) method, using a 1.2 x 4.0 mm glass column packed with 3% G3 on S1AB with helium as a carrier gas. If the column has been contaminated with very hydrophobic material, wash the column over night with 25% 2-propanol in distilled water at a flow rate of 0.2mL/min. Phase: • Non-bonded • Poly(biscyanopropyl siloxane) 3.9 mm x 15 cm, 4 µm, manufacturer Waters. HPLC Method Development Services. HPLC Column Stainless Steel Inner Diameter: 4,6 mm Outer Diameter: 8,0 mm Bed Length: 100 mm Total Length: 140 mm prepacked with ChromBudget 120-5-C18 Matrix: Silica, High Purity, Spherical Average Particle Size: 5 µm Average Pore Size: 120 Å Surface Area: 300 m2 / gr Phase: Reversed Phase, RP-Phase Ligand: C18 Bonding: covalent bi-functional Columns with all non-metallic wetted flow-paths for potentially corrosive processes, for Chiralscreen is easy to use kit from Daicel enzyme library to obtain chiral alcohols from corresponding ketones. Not official USP-NF text; ESOMEPRAZOLE MAGNESIUM: PF: LGS# Brand: Type of Test: Additional Information: 32(3) L1: Nova-Pak C18: Assay & Chrom. column. If you are running USP<401>, your acids are in the methyl ester form and a G16 column works fine. For additional applications, please ask for our Chiral Users Guide. ChromGC™ CH-STAR-WAX. ... Hi Chrom Reversible ODS. Part of the arch sciences group Paper and thin-layer chromatography are ordinarily more … Please note: The USP L-41 column is the CHIRAL-AGP. (2)Useful for separating highly hydrophilic compounds including saccharides, nucleic acids and organic acids. Stable from pH 1-14. What brand of HPLC/GC column was used in the development and/or validation of a particular test? Choose the speed, efficiency, and long-lasting ruggedness of the Raptor C18. Enantiomers are separated based on the number and type of each interaction that occurs during their exposure to the chiral stationary phase. Hyperclone BDS C18. USP Code: This column meets USP G5 requirements. When you need a general-purpose LC column, don't just grab any C18. Daicel also … Low-Carbon C18 column (1)Compatible with aqueous mobile phases including 100% H2O. (2)The first choice for a wide variety of analyses. Equivalent to USP G14, G15, G16. Helix Chromatography is pioneering HPLC with new mixed-mode columns. Nucleogel Sugar Ca Nucleogel Sugar 810 Ca Rezex RCM-Monosaccharide Sucrose, fosfomycin, sodium and tris are very polar in nature. Compared to a conventional column, AxiChrom columns' purposeful design makes process chromatography easier, safer, and … USP L## packings for HPLC columns. Welcome to Access Point! ), offer automated column packing that enables fast start-up, reproducible results, and maximized uptime. Crawford Scientific Ltd Holm Street Strathaven Lanarkshire ML10 6NB. Sucrose, fosfomycin, sodium and TRIS are part of the fosfomycin formulation. HxSil C18. Is there an alternative chromatographic column for a particular test? HP-C18. Hamilton HC-75 Ca. VertiSep™ MMS C8/NH2 is recommended for USP L28. I use the FFAP columns when I need to analyze fatty acids in their acid form. L1. General description Application: This highly polar biscyanopropyl column was specifically designed for detailed separation of geometricpositional (cis/trans) isomers of fatty acid methyl esters (FAMEs).It is extremely effective for FAME isomer applications. The USP (United States Pharmacopeia) uses this method. 32(3) L7: LiChrosorb RP-8: Assay & Chrom. Use your USP Access Point login credentials to register for events and courses, access your subscriptions to USP's free resources, applications, and more. Hichrom C18. Chrom Tech is a HPLC column supplier and an authorized distributor for Agilent Technologies, Concise Separations, Hamilton, Imtakt USA, Jordi Labs, Regis, Restek, Millipore Sigma, Thermo Scientific, HiChrom, and more. CARBOSep COREGEL-87C CARBOSep L19. Benson BC-200. > back to SiliaChrom Plus HPLC Portfolio. HPLC COLUMNS AND UHPLC COLUMNS. LC Column Selection – USP Specifications 58 For free technical advice and support email technical@hichrom.co.uk HPLC COLUMN SELECTION BY USP SPECIFICATIONS USP Specifications The following list of USP (United States Pharmacopoeia) column specifications (USP 35) includes a selection of recommended columns within each category. Phone: +1 732 380 8900 Fax: +1 910 769 9435 Toll-free USA: 1 888 248 4972 Click this link to Email Typically chromatographers must choose HPLC columns by comparing the surface area and carbon load data supplied by the column manufacturers. HyperREZ XP Carbohydrate Ca HyperREZ XP Sugar Alcohols MCI GEL CK08EC. Simultaneous HPLC Analysis of Sucrose, Fosfomycin and Common Counterions on Amaze TH Mixed-Mode Column. Our R&D works to continually enhance our portfolio to suit customer’s requirements and offer unique and powerful new products. purity: Alternative column. This results in small N values when peak overlap is large. The AxiChrom column platform (Fig 1) is a revolutionary concept in column chromatography. Install the best GC columns into your instrument. The FFAP stationary phase is modified with acid to provide a very inert column that can accommodate the demanding analysis of acids dissolved in water. This traditional end-capped C18 offers the highest hydrophobic retention of any Raptor phase, and it is compatible with a wide range of mobile phases from moderately acidic to neutral (pH 2–8). requirements of USP Class VI as described in USP Biological Reactivity Tests In Vivo <88>, 21 CFR Part 177. MANUFACTURER/ VENDOR. It allows rapid screening without any special skill. This is followed by C8-bonded silica (L7), pure silica (L3), cyano-bonded silica (L10) and phenyl-bonded silica (L11). Find the USP of the column with “control + F ” . VertiSep™ MMS C8/C18 column is multifunctional phase of C8 and C18 groups for the reversed phase separation of basic molecules. Chrom-Clone HPLC Information Make the switch to Chrom-Clone, a cost-effective, guaranteed direct replacement to Kromasil® based columns. Preparative Chromatography. If your analytes are in the acid form, a G25 or G35 is preffered. The FFAP columns are better deactivated for acids and should give less tailing and better precision. 72003AST: Astec ® CHIRALDEX ® A-DA Capillary GC Column L × I.D. Chrom-Clone columns are packed and manufactured only by Phenomenex, thereby reducing the chance of column-to-column variation. Standard C18 column (1)Fully end-capping for complete coverage of the silica surface. L. PACKING. Pressure-retaining and wetted parts are traceable to batch level. Greater Wilmington, NC USA. Hamilton HC-40. Hypersil 100 C18. Other Column Brands. Hydrosphere C18. purity: 4.0 mm x 12.5 mm, 5 µm, manufacturer Merck KGaA. Column comparison function (F) The Fs value of this column relative to the one you have selected. Agilent J&W GC columns have the widest range of standard, GC/MS, and Ultra Inert stationary phases to deliver consistent column inertness and exceptionally low column bleed with high upper temperature limits, ensuring accurate peak identification and quantification for your gas chromatography analysis. HPLC Column Selection: A Comparison of Alltech C18 and C8 Columns Alltech has developed a method for choosing C18 and C8 columns based on peak capacities and column selectivity of polar and nonpolar compounds. The most popular column is an octadecyl carbon chain (C18)-bonded silica (USP classification L1). This also presents a problem if the peak is distorted, so that it has multiple inflection points. The types of chromatography useful in qualitative and quantitative analysis that are employed in the USP procedures are column, gas, paper, thin-layer, (including high-performance thin-layer chromatography), and pressurized liquid chromatography (commonly called high-pressure or high-performance liquid chromatography). Not as hydrophobic as typical C18-silica phases, so less organic solvent is required. Agilent J&W HP-FFAP is designed primarily for the analysis of organic acids, free fatty acids, or samples that require quantitation of acidic impurities. CarboSep CHO-820. The Fs value is the basis for ranking the similarity/difference between two columns. They are free from animal-derived components or in compliance with (EMEA/410/01 Rev.2). Bookmark this page (Ctrl-D) for easy access. Buy Heritage HPLC Columns from the manufacturer in Chicago. Fs is essentially the distance separating two columns in a six-dimensional parameter space. Hi-Plex Ca. Carbomix Ca-NP CARBOSep CHO620. AxiChrom 50 to 200 mm chromatography columns. ・・・1) 2. The use of highly volatile diluent dichloromethane is required for sample preparation. Contact Us. VertiSep™ MMS C8/C18 offers more excellent gradient separation to C18 or C8 only for applications especially pharmaceutical applications and recommended for USP L42. Chiral chromatography refers to the separation of enantiomers using a chiral HPLC column, an HPLC column that is packed with a chiral stationary phase (CSP). It simplifies column handling at all scales, from pilot to large-scale production. SiliCycle offers a wide range of chromatographic selectivities to HPLC end-users. Hi-Plex Ca USP. ZirChrom ®-PBD (USP L49) columns offer today's chromatographer the following advantages: A reversed-phase like material, but with slightly different selectivity. HydroBond PS-C18. Aminex Cabohydrate HPX-87C Benson BC-100. Best of all, each GC column that leaves our facility comes with an unbeatable guarantee: Restek Pure Satisfaction. See page 80 for ordering information.
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